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This function performs quantile normalization on high-dimensional cytometry data in tidy format using linear rescaling. Each channel specified by `channel_cols` is rescaled such that the maximum value is 1 and the minimum value is 0. `group_cols` specifies the columns that should be used to break cells into groups in which the rescaling should be performed separately.

Usage

tof_batch_correct_rescale(tof_tibble, channel_cols, group_cols, augment = TRUE)

Arguments

tof_tibble

A `tof_tbl` or a `tibble`.

channel_cols

Unquoted column names representing columns that contain single-cell protein measurements. Supports tidyselect helpers.

group_cols

Optional. Unquoted column names indicating which columns should be used to group cells before batch correction. Batch correction is then performed independently within each group. Supports tidyselect helpers.

augment

A boolean value indicating if the output should replace the `channel_cols` in `tof_tibble` with the new, batch corrected columns (TRUE, the default) or if it should only return the batch-corrected columns (FALSE) with all other columns omitted.

Value

If augment = TRUE, a tibble with the same number of rows and columns as tof_tibble, with the columns specified by `channel_cols` batch-corrected. If augment = FALSE, a tibble containing only the batch-corrected `channel_cols`.

Examples

NULL
#> NULL